Raw Data for BglB Variant D63M

Expression Data

Kinetic Assay Data

slope of D63M A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0	0
	B	25.00		0.00035	4.00E-04
	C	8.33		0	0
	D	2.78	0	0	0
	E	0.93	0.0004	3.50E-04
	F	0.31	0	5.50E-04	0.00035
	G	0.10	0	0
	H	0.03	0.0004	0

Yield (mg/mL): 0.618     Dilution Factor:  10×

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Protein purified on 5/19/2022 and assayed on 5/26/2022.
Data uploaded by and last updated on 2022-06-27 07:04:03.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.81E-01	1.99E-01	1.87E-01
	B	25.00	1.52E-01	1.20E-01	1.77E-01
	C	8.33	1.18E-01	1.45E-01	1.04E-01
	D	2.78	9.41E-02	9.41E-02	7.95E-02
	E	0.93	3.78E-02	4.10E-02	3.09E-02
	F	0.31	1.36E-02	1.65E-02	1.40E-02
	G	0.10	5.30E-03	4.45E-03	5.15E-03
	H	0.03	2.50E-04	2.50E-04

Yield (mg/mL): 1.76     Dilution Factor:  100×

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Protein purified on 4/18/22 and assayed on 4/20/22.
Data uploaded by bridget5 and last updated on 2023-01-11 14:39:36.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.