Raw Data for BglB Variant W120C

Expression Data

Yield (mg/mL): 0.19

Kinetic Assay Data

slope of W120C A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.0011	0.00125	0.00135
	B	25.00	0.0006	0.0005	0.0004
	C	8.33	0.0003	0.0008	0.0006
	D	2.78	0.00045	0.00045
	E	0.93		0	0
	F	0.31	0.0002	0.00025	0.0003
	G	0.10	0.0003	0.00025	0.00035
	H	0.03		0	0

Yield (mg/mL): 0.19     Dilution Factor:  10×

Download these data

Protein purified on 5/18/2022 and assayed on 5/19/2022.
Data uploaded by and last updated on 2022-06-27 07:04:03.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of W120C A_{420 nm}

		T (°C)	1	2	3
	A	50.0	0.0003	0	0.0009
	B	48.3	0.0007	0.0005	0
	C	45.7	0.0004		0.0005
	D	42.4	0.0005		0.0006
	E	37.7		0.0005	0.0005
	F	33.6	0.0006	0.0005	0.0005
	G	31.3	0.0006	0.0003	0.0005
	H	30.0	0.0007	0.0006	0.0008

Download these data

Protein purified on 5/18/2022 and assayed on 5/19/2022.
Data uploaded by mspiridonova and last updated on 2022-06-27 07:10:33.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.