D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant D35W

Expression Data

Yield (mg/mL): 0.457

Kinetic Assay Data

**slope of D35W A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.018	0.01895	0.0179
B	25.00		0.01575	0.0149
C	8.33	0.0113	0.0113	0.009601
D	2.78	0.00905	0.01155	0.01225
E	0.93	0.0038		0.00405
F	0.31		0.0015	0.0014
G	0.10	0.0004	0.00045	0.00055
H	0.03	0	0

Yield (mg/mL): 0.457 Dilution Factor: 10×

Protein purified on 5/9/22 and assayed on 5/12/22.
Data uploaded by clairehuntington and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.07E-01	8.71E-01	8.76E-01
B	25.00	7.51E-01	8.07E-01	8.13E-01
C	8.33	5.88E-01	6.61E-01	6.45E-01
D	2.78	4.07E-01	3.27E-01	3.94E-01
E	0.93	1.56E-01	1.23E-01	1.80E-01
F	0.31	6.61E-02	4.79E-02	7.30E-02
G	0.10	2.47E-02		2.52E-02
H	0.03	8.50E-04	6.00E-04	7.50E-04

Yield (mg/mL): 3.557 Dilution Factor: 100×

Protein purified on and assayed on .
Data uploaded by clairehuntington and last updated on 2023-11-07 18:27:52.

Design Data

Rosetta Score for D35W: 14.7

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.