D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T218Q

Expression Data

Yield (mg/mL): 1.042

Kinetic Assay Data

**slope of T218Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.34E-02		1.34E-02
B	25.00	5.75E-03	5.50E-03	6.90E-03
C	8.33	2.65E-03		2.85E-03
D	2.78	1.30E-03		1.25E-03
E	0.93	6.00E-04	5.50E-04
F	0.31	3.00E-04	4.00E-04	2.50E-04
G	0.10	3.00E-04	2.50E-04	2.00E-04
H	0.03	2.50E-04		2.50E-04

Yield (mg/mL): 1.042 Dilution Factor: 10×

Protein purified on 4/18/22 and assayed on 4/18/22.
Data uploaded by bridget5 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.81E-01	1.99E-01	1.87E-01
B	25.00	1.52E-01	1.20E-01	1.77E-01
C	8.33	1.18E-01	1.45E-01	1.04E-01
D	2.78	9.41E-02	9.41E-02	7.95E-02
E	0.93	3.78E-02	4.10E-02	3.09E-02
F	0.31	1.36E-02	1.65E-02	1.40E-02
G	0.10	5.30E-03	4.45E-03	5.15E-03
H	0.03	2.50E-04	2.50E-04

Yield (mg/mL): 1.76 Dilution Factor: 100×

Protein purified on 4/18/22 and assayed on 4/20/22.
Data uploaded by bridget5 and last updated on 2023-01-11 14:39:36.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.