Raw Data for BglB Variant K61H

Expression Data

SDS-PAGE
 6.161

Kinetic Assay Data

slope of K61H A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.26E-01 3.10E-01 3.03E-01
B 25.00 2.63E-01 2.81E-01 2.53E-01
C 8.33 2.26E-01 2.15E-01 2.30E-01
D 2.78 1.92E-01 1.69E-01 1.91E-01
E 0.93 9.69E-02 1.06E-01 9.81E-02
F 0.31 4.49E-02 4.05E-02 5.12E-02
G 0.10 1.34E-02 1.37E-02 1.58E-02
H 0.03 0 0
 6.161      100×

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by rubicarla and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.93E-01 3.35E-01
B 25.00 3.26E-01 3.34E-01
C 8.33 2.19E-01 1.78E-01 2.04E-01
D 2.78 1.48E-01 1.28E-01 1.48E-01
E 0.93 6.06E-02 5.24E-02 6.08E-02
F 0.31 1.38E-02 2.81E-02 2.39E-02
G 0.10 1.14E-02 1.22E-02
H 0.03 4.50E-04 6.00E-04 6.00E-04
 5.603      100×

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by jnune033 and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of K61H A420 nm
T (°C) 1 2 3
A 50.0 0 0 0
B 48.0 0 0
C 46.0 1.80E-03 1.50E-03
D 42.0 1.50E-02 1.50E-02 1.50E-02
E 38.0 9.75E-01 9.98E-01 8.55E-01
F 34.0 1.62E+00 1.71E+00
G 31.0 1.52E+00 1.76E+00 1.53E+00
H 30.0 1.62E+00 1.66E+00 1.88E+00

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by rubicarla and last updated on 2022-06-27 07:10:33.


Reference Data for WT

slope of K61H A420 nm
T (°C) 1 2 3
A 30.0 2.078 1.876 1.811
B 31.0 1.58E+00 1.58E+00 1.50E+00
C 34.0 1.52E+00 1.56E+00 1.53E+00
D 38.0 1.37E+00 1.29E+00 1.20E+00
E 42.0 4.98E-02 4.92E-02 4.71E-02
F 46.0 2.10E-03 1.50E-03 1.80E-03
G 48.0 1.80E-03 1.50E-03 1.50E-03
H 50.0 1.50E-03 1.20E-03 1.50E-03

Protein purified on and assayed on .
Data uploaded by jnune033 and last updated on 2022-06-27 07:10:33.


Design Data

3.33

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.