Raw Data for BglB Variant K61Y
Expression Data
0.4676
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 1.35E-02 | 1.37E-02 | 1.28E-02 | ||
| B | 25.00 | 1.11E-02 | 1.20E-02 | 1.22E-02 | ||
| C | 8.33 | 1.01E-02 | 9.60E-03 | 9.45E-03 | ||
| D | 2.78 | 7.95E-03 | 8.85E-03 | 7.65E-03 | ||
| E | 0.93 | 4.65E-03 | 4.05E-03 | 4.20E-03 | ||
| F | 0.31 | 2.10E-03 | 1.80E-03 | 2.10E-03 | ||
| G | 0.10 | 9.00E-04 | 1.05E-03 | 1.05E-03 | ||
| H | 0.03 | 4.50E-04 | 4.50E-04 |
Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by Nfadh001 and last updated on 2022-06-27 07:04:03.
Reference Data for WT
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 3.02E-01 | 2.96E-01 | 2.99E-01 | ||
| B | 25.00 | 2.30E-01 | 2.39E-01 | 2.38E-01 | ||
| C | 8.33 | 1.76E-01 | 1.79E-01 | 2.08E-01 | ||
| D | 2.78 | 1.28E-01 | 1.27E-01 | 1.33E-01 | ||
| E | 0.93 | 6.66E-02 | 6.51E-02 | 7.64E-02 | ||
| F | 0.31 | 3.32E-02 | 3.17E-02 | 3.86E-02 | ||
| G | 0.10 | 1.25E-02 | 1.14E-02 | 1.64E-02 | ||
| H | 0.03 | 0 | 0 |
Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by jnune033 and last updated on 2022-06-27 07:04:03.
Thermostability Assay Data
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 0 | 0 | |||
| B | 48.0 | 1.20E-03 | 9.00E-04 | |||
| C | 46.0 | 1.20E-03 | 1.20E-03 | 1.20E-03 | ||
| D | 42.0 | 3.00E-03 | 3.60E-03 | 3.00E-03 | ||
| E | 38.0 | 5.52E-02 | 6.30E-02 | 7.11E-02 | ||
| F | 34.0 | 1.08E-01 | 1.08E-01 | 1.09E-01 | ||
| G | 31.0 | 9.12E-02 | 1.08E-01 | 8.19E-02 | ||
| H | 30.0 | 1.26E-01 | 1.53E-01 | 7.71E-02 |
Reference Data for WT
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 30.0 | 2.078 | 1.876 | 1.811 | ||
| B | 31.0 | 1.58E+00 | 1.58E+00 | 1.50E+00 | ||
| C | 34.0 | 1.52E+00 | 1.56E+00 | 1.53E+00 | ||
| D | 38.0 | 1.37E+00 | 1.29E+00 | 1.20E+00 | ||
| E | 42.0 | 4.98E-02 | 4.92E-02 | 4.71E-02 | ||
| F | 46.0 | 2.10E-03 | 1.50E-03 | 1.80E-03 | ||
| G | 48.0 | 1.80E-03 | 1.50E-03 | 1.50E-03 | ||
| H | 50.0 | 1.50E-03 | 1.20E-03 | 1.50E-03 |
Protein purified on and assayed on .
Data uploaded by jnune033 and last updated on 2022-06-27 07:10:33.
Design Data
Citations
The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.