Raw Data for BglB Variant K61D

Expression Data

SDS-PAGE
 1.107

Kinetic Assay Data

slope of K61D A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.81E-02 3.87E-02 3.83E-02
B 25.00 3.27E-02 3.20E-02 2.67E-02
C 8.33 2.48E-02 2.61E-02
D 2.78 1.59E-02 1.55E-02 1.70E-02
E 0.93 7.50E-03 8.40E-03 8.25E-03
F 0.31 3.00E-03 3.00E-03 3.30E-03
G 0.10 1.50E-03 1.65E-03 1.50E-03
H 0.03 0 0
 1.107      100×

Protein purified on 3/25/22 and assayed on 4/1/22.
Data uploaded by Josemontero and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.02E-01 2.96E-01 2.99E-01
B 25.00 2.30E-01 2.39E-01 2.38E-01
C 8.33 1.76E-01 1.79E-01 2.08E-01
D 2.78 1.28E-01 1.27E-01 1.33E-01
E 0.93 6.66E-02 6.51E-02 7.64E-02
F 0.31 3.32E-02 3.17E-02 3.86E-02
G 0.10 1.25E-02 1.14E-02 1.64E-02
H 0.03 0 0
 5.445      100×

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by jnune033 and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of K61D A420 nm
T (°C) 1 2 3
A 50.0 0 0 0
B 48.0 1.20E-03 9.00E-04
C 46.0 0 0
D 42.0 3.90E-03 3.60E-03 3.00E-03
E 38.0 1.31E-01 1.22E-01
F 34.0 1.98E-01 1.80E-01 1.89E-01
G 31.0 2.41E-01 2.48E-01 2.17E-01
H 30.0 1.80E-01 1.94E-01 1.96E-01

Protein purified on 3/25/22 and assayed on 4/1/22.
Data uploaded by Josemontero and last updated on 2022-06-27 07:10:33.


Reference Data for WT

slope of K61D A420 nm
T (°C) 1 2 3
A 30.0 2.078 1.876 1.811
B 31.0 1.58E+00 1.58E+00 1.50E+00
C 34.0 1.52E+00 1.56E+00 1.53E+00
D 38.0 1.37E+00 1.29E+00 1.20E+00
E 42.0 4.98E-02 4.92E-02 4.71E-02
F 46.0 2.10E-03 1.50E-03 1.80E-03
G 48.0 1.80E-03 1.50E-03 1.50E-03
H 50.0 1.50E-03 1.20E-03 1.50E-03

Protein purified on and assayed on .
Data uploaded by jnune033 and last updated on 2022-06-27 07:10:33.


Design Data

5.59

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.