Raw Data for BglB Variant K61E

Expression Data

SDS-PAGE
 1.357

Kinetic Assay Data

slope of K61E A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 5.67E-02 5.39E-02 5.57E-02
B 25.00 4.55E-02 5.18E-02 4.86E-02
C 8.33 3.68E-02 4.11E-02 3.69E-02
D 2.78 2.37E-02 2.28E-02 2.52E-02
E 0.93 1.19E-02 1.14E-02 1.20E-02
F 0.31 6.45E-03 9.60E-03 4.20E-03
G 0.10 2.55E-03 2.70E-03 2.85E-03
H 0.03 0 0
 1.357      100×

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by ccast302 and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.93E-01 3.35E-01
B 25.00 3.26E-01 3.34E-01
C 8.33 2.19E-01 1.78E-01 2.04E-01
D 2.78 1.48E-01 1.28E-01 1.48E-01
E 0.93 6.06E-02 5.24E-02 6.08E-02
F 0.31 1.38E-02 2.81E-02 2.39E-02
G 0.10 1.14E-02 1.22E-02
H 0.03 4.50E-04 6.00E-04 6.00E-04
 5.603      100×

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by jnune033 and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of K61E A420 nm
T (°C) 1 2 3
A 50.0 0 0
B 48.0 0 0
C 46.0 1.80E-03 9.00E-04 0
D 42.0 6.30E-03 3.60E-03 4.80E-03
E 38.0 2.27E-01 2.10E-01
F 34.0 2.81E-01 2.71E-01 3.30E-01
G 31.0 2.89E-01 3.05E-01 2.84E-01
H 30.0 3.06E-01 3.13E-01 3.10E-01

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by ccast302 and last updated on 2022-06-27 07:10:33.


Reference Data for WT

slope of K61E A420 nm
T (°C) 1 2 3
A 30.0 2.113 1.912 2.009
B 31.0 1.85E+00 1.78E+00 1.51E+00
C 34.0 1.28E+00 1.58E+00 1.71E+00
D 38.0 1.30E+00 1.08E+00 1.38E+00
E 42.0 4.86E-02 4.77E-02 4.44E-02
F 46.0 1.80E-03 0 2.70E-03
G 48.0 1.20E-03 1.20E-03 1.20E-03
H 50.0 1.50E-03 1.80E-03

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by jnune033 and last updated on 2022-06-27 07:10:33.


Design Data

0.81

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.