Raw Data for BglB Variant K61Q

Expression Data

SDS-PAGE
 1.418

Kinetic Assay Data

slope of K61Q A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.22E-02 6.71E-02
B 25.00 5.58E-02 5.63E-02
C 8.33 1.71E-02 2.90E-02 3.84E-02
D 2.78 2.61E-02 2.51E-02 3.20E-02
E 0.93 1.50E-02 1.43E-02
F 0.31 3.15E-03 3.75E-03
G 0.10 1.65E-03 2.25E-03 2.25E-03
H 0.03 0 0 0
 1.418      100×

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by AlyxandLauren and last updated on 2024-05-23 10:04:09.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.93E-01 3.35E-01
B 25.00 3.26E-01 3.34E-01
C 8.33 2.19E-01 1.78E-01 2.04E-01
D 2.78 1.48E-01 1.28E-01 1.48E-01
E 0.93 6.06E-02 5.24E-02 6.08E-02
F 0.31 1.38E-02 2.81E-02 2.39E-02
G 0.10 1.14E-02 1.22E-02
H 0.03 4.50E-04 6.00E-04 6.00E-04
 5.603      100×

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by jnune033 and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of K61Q A420 nm
T (°C) 1 2 3
A 50.0 1.20E-03 1.20E-03
B 48.0 1.20E-03 1.20E-03
C 46.0 1.50E-03 1.50E-03 1.20E-03
D 42.0 1.05E-02 1.02E-02 9.30E-03
E 38.0 2.17E-01 2.59E-01 2.45E-01
F 34.0 2.68E-01 2.71E-01 2.55E-01
G 31.0 2.69E-01 2.94E-01 2.90E-01
H 30.0 2.93E-01 2.95E-01 2.99E-01

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by AlyxandLauren and last updated on 2022-06-27 07:10:33.


Reference Data for WT

slope of K61Q A420 nm
T (°C) 1 2 3
A 30.0 2.113 1.912 2.009
B 31.0 1.85E+00 1.78E+00 1.51E+00
C 34.0 1.28E+00 1.58E+00 1.71E+00
D 38.0 1.30E+00 1.08E+00 1.38E+00
E 42.0 4.86E-02 4.77E-02 4.44E-02
F 46.0 1.80E-03 0 2.70E-03
G 48.0 1.20E-03 1.20E-03 1.20E-03
H 50.0 1.50E-03 1.80E-03

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by jnune033 and last updated on 2022-06-27 07:10:33.


Design Data

0.82

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.