Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.02E-01	2.96E-01	2.99E-01
	B	25.00	2.30E-01	2.39E-01	2.38E-01
	C	8.33	1.76E-01	1.79E-01	2.08E-01
	D	2.78	1.28E-01	1.27E-01	1.33E-01
	E	0.93	6.66E-02	6.51E-02	7.64E-02
	F	0.31	3.32E-02	3.17E-02	3.86E-02
	G	0.10	1.25E-02	1.14E-02	1.64E-02
	H	0.03	0	0

Yield (mg/mL): 5.445     Dilution Factor:  100×

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Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by jnune033 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm}

		T (°C)	1	2	3
	A	30.0	2.113	1.912	2.009
	B	31.0	1.85E+00	1.78E+00	1.51E+00
	C	34.0	1.28E+00	1.58E+00	1.71E+00
	D	38.0	1.30E+00	1.08E+00	1.38E+00
	E	42.0	4.86E-02	4.77E-02	4.44E-02
	F	46.0	1.80E-03	0	2.70E-03
	G	48.0	1.20E-03	1.20E-03	1.20E-03
	H	50.0	1.50E-03		1.80E-03

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Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by jnune033 and last updated on 2022-06-27 07:10:33.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.