D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W407Y

Expression Data

Yield (mg/mL): 1.094

Kinetic Assay Data

**slope of W407Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	9.00E-04	9.00E-04	9.00E-04
B	25.00	6.00E-04	7.50E-04	1.20E-03
C	8.33	6.00E-04	7.50E-04	7.50E-04
D	2.78	7.50E-04	9.00E-04
E	0.93	7.50E-04	7.50E-04
F	0.31
G	0.10		6.00E-04	7.50E-04
H	0.03		6.00E-04	7.50E-04

Yield (mg/mL): 1.094 Dilution Factor: 10×

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by and last updated on 2024-08-01 15:05:34.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.87E-02		7.40E-02
B	25.00	6.84E-02		4.56E-02
C	8.33	6.80E-02	5.37E-02	4.14E-02
D	2.78	4.89E-02	4.07E-02	3.12E-02
E	0.93		2.07E-02	1.80E-02
F	0.31	1.37E-02	9.60E-03	7.95E-03
G	0.10		3.15E-03	3.30E-03
H	0.03	7.50E-04	7.50E-04

Yield (mg/mL): 1.36 Dilution Factor: 100×

Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by carimaceo and last updated on 2023-09-11 10:28:44.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of W407Y A_{420 nm}**
	T (°C)	1	2	3
A	50.0	1.50E-03	1.20E-03	1.20E-03
B	48.3	9.00E-04		1.20E-03
C	45.7	1.20E-03	1.20E-03	1.20E-03
D	42.4	2.19E-02	2.34E-02
E	37.7	4.00E-01	3.08E-01	4.01E-01
F	33.6	3.56E-01	3.61E-01	3.46E-01
G	31.3	3.66E-01	3.83E-01	3.72E-01
H	30.0	3.76E-01	4.10E-01	3.71E-01

Protein purified on 3/15/2022 and assayed on 4/1/2022.
Data uploaded by carimaceo and last updated on 2022-06-27 07:10:33.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.