Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	9.23E-02	9.06E-02	9.47E-02
	B	25.00	7.86E-02	7.88E-02	7.98E-02
	C	8.33	7.07E-02	7.04E-02	6.66E-02
	D	2.78	6.69E-02	4.83E-02	7.08E-02
	E	0.93	2.36E-02	2.22E-02	2.37E-02
	F	0.31	9.00E-03	9.15E-03	9.30E-03
	G	0.10	3.90E-03	3.45E-03	3.75E-03
	H	0.03	6.00E-04	4.50E-04	4.50E-04

Yield (mg/mL): 2.032     Dilution Factor:  100×

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Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by lalma020 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm}

		T (°C)	1	2	3
	A	50.0		9.00E-04	1.20E-03
	B	48.0	9.00E-04		1.20E-03
	C	46.0	1.50E-03	1.50E-03	1.20E-03
	D	42.0	3.63E-02	3.78E-02	3.63E-02
	E	38.0	4.47E-01	4.43E-01	3.87E-01
	F	34.0	4.43E-01	4.53E-01	4.54E-01
	G	31.0	4.52E-01	4.81E-01	4.88E-01
	H	30.0	4.81E-01	5.17E-01	5.03E-01

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Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by lalma020 and last updated on 2022-06-27 07:10:33.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.