Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.93E-01		3.35E-01
	B	25.00	3.26E-01		3.34E-01
	C	8.33	2.19E-01	1.78E-01	2.04E-01
	D	2.78	1.48E-01	1.28E-01	1.48E-01
	E	0.93	6.06E-02	5.24E-02	6.08E-02
	F	0.31	1.38E-02	2.81E-02	2.39E-02
	G	0.10	1.14E-02	1.22E-02
	H	0.03	4.50E-04	6.00E-04	6.00E-04

Yield (mg/mL): 5.603     Dilution Factor:  100×

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Protein purified on 3/25/2022 and assayed on 4/1/2022.
Data uploaded by jnune033 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm}

		T (°C)	1	2	3
	A	30.0	2.078	1.876	1.811
	B	31.0	1.58E+00	1.58E+00	1.50E+00
	C	34.0	1.52E+00	1.56E+00	1.53E+00
	D	38.0	1.37E+00	1.29E+00	1.20E+00
	E	42.0	4.98E-02	4.92E-02	4.71E-02
	F	46.0	2.10E-03	1.50E-03	1.80E-03
	G	48.0	1.80E-03	1.50E-03	1.50E-03
	H	50.0	1.50E-03	1.20E-03	1.50E-03

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Protein purified on and assayed on .
Data uploaded by jnune033 and last updated on 2022-06-27 07:10:33.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.