D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N160A

Expression Data

Yield (mg/mL): 3.586

Kinetic Assay Data

**slope of N160A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3457	0.3544	0.3729
B	25.00	0.2841	0.2889	0.2761
C	8.33	0.1393	0.1211	0.1374
D	2.78	0.1026	0.07450	0.09095
E	0.93	0.04445	0.03235	0.03740
F	0.31	0.03060	0.02325	0.02120
G	0.10	0.008951	0.007450	0.006500
H	0.03		0.0004000	0.0003500

Yield (mg/mL): 3.586 Dilution Factor: 100×

Protein purified on 2/28/2022 and assayed on 3/2/2022.
Data uploaded by and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2764	0.2756	0.2734
B	25.00	0.2308	0.2895	0.2651
C	8.33	0.1917	0.2001	0.2177
D	2.78	0.1255	0.1333	0.1307
E	0.93	0.08420	0.08451	0.05905
F	0.31	0.04300	0.04270
G	0.10	0.01750	0.01700	0.01360
H	0.03	0.001100		0.0007001

Yield (mg/mL): 4.166 Dilution Factor: 100×

Protein purified on 2/28/2022 and assayed on 3/1/2022.
Data uploaded by nikchupkin and last updated on 2022-06-27 07:10:33.

Design Data

Rosetta Score for N160A: 2.41

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.