D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W409R

Expression Data

Yield (mg/mL): 3.386

Kinetic Assay Data

**slope of W409R A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.09010	0.08965	0.08945
B	25.00	0.08460	0.08460	0.08690
C	8.33	0.05595	0.06495	0.05745
D	2.78	0.04600	0.04890	0.03960
E	0.93	0.03315	0.02915	0.02060
F	0.31	0.01755	0.01295	0.01555
G	0.10	0.006300	0.005000	0.004900
H	0.03	0.001200	0.0004500	0

Yield (mg/mL): 3.386 Dilution Factor: 100×

Protein purified on 2/28/2022 and assayed on 3/2/2022.
Data uploaded by nikchupkin and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2764	0.2756	0.2734
B	25.00	0.2308	0.2895	0.2651
C	8.33	0.1917	0.2001	0.2177
D	2.78	0.1255	0.1333	0.1307
E	0.93	0.08420	0.08451	0.05905
F	0.31	0.04300	0.04270
G	0.10	0.01750	0.01700	0.01360
H	0.03	0.001100		0.0007001

Yield (mg/mL): 4.166 Dilution Factor: 100×

Protein purified on 2/28/2022 and assayed on 3/1/2022.
Data uploaded by nikchupkin and last updated on 2022-06-27 07:10:33.

Design Data

Rosetta Score for W409R: 2.59

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.