Raw Data for BglB Variant A306Q

Expression Data

SDS-PAGE
 2.08

Kinetic Assay Data

slope of A306Q A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2926 0.3690 0.4306
B 25.00 0.2911 0.3241 0.2608
C 8.33 0.2272 0.2532 0.2643
D 2.78 0.1447 0.1536 0.1906
E 0.93 0.05555 0.07465 0.08940
F 0.31 0.02230 0.02650 0.03040
G 0.10 0.008150 0.009100
H 0.03 0.003150 0.003350 0.004300
 2.08      100×

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by jsanch and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3042 0.2949 0.2858
B 25.00 0.2766 0.2821 0.2964
C 8.33 0.1958 0.2027 0.1592
D 2.78 0.1121 0.1206 0.1524
E 0.93 0.04915 0.04830 0.05810
F 0.31 0.01970 0.01795 0.02290
G 0.10 0.006900 0.007300 0.008600
H 0.03 0.003100 0.003000
 1.546      100×

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by WilliamBenfield and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of A306Q A420 nm
T (°C) 1 2 3
A 50.0 0.001000 0.001000 0.0008000
B 48.0 0.001700 0.001900 0.002200
C 46.0 0.03080 0.02730 0.02520
D 42.0 0.2403 0.2391 0.2599
E 38.0 0.7701 0.8405 0.9180
F 34.0 1.004 1.057 1.182
G 31.0 1.100 1.118 1.057
H 30.0 1.221 1.190

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by jsanch and last updated on 2022-06-27 07:10:33.


Reference Data for WT

slope of A306Q A420 nm
T (°C) 1 2 3
A 50.0 0.001200 0.001300
B 48.0 0.001400 0.001100
C 46.0 0.005200 0.003700
D 42.0 0.2682 0.2858
E 38.0 0.7912 1.175 1.422
F 34.0 1.520 1.533 1.458
G 31.0 1.662 1.639 1.649
H 30.0 1.761 1.707 1.718

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by WilliamBenfield and last updated on 2022-06-27 07:10:33.


Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.