Raw Data for BglB Variant A306Q

Expression Data

SDS-PAGE
 2.08

Kinetic Assay Data

Plate kinetic-digestase2

slope of A306Q A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2926 0.369 0.4306
B 25.00 0.2911 0.3241 0.2608
C 8.33 0.2272 0.2532 0.2643
D 2.78 0.1447 0.1536 0.1906
E 0.93 0.05555 0.07465 0.0894
F 0.31 0.0223 0.0265 0.0304
G 0.10 0.00815 0.0091
H 0.03 0.00315 0.00335 0.0043
 2.08      100×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3042 0.2949 0.2858
B 25.00 0.2766 0.2821 0.2964
C 8.33 0.1958 0.2027 0.1592
D 2.78 0.1121 0.1206 0.1524
E 0.93 0.04915 0.0483 0.0581
F 0.31 0.0197 0.01795 0.0229
G 0.10 0.0069 0.0073 0.0086
H 0.03 0.0031 0.003
 1.546      100×

Protein purified on 11/4/21 and assayed on 11/18/21.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of A306Q A420 nm
T (°C) 1 2 3
A 50.0 0.0006000 0.0006001 0.0007000
B 48.0 0.001000 0.001100
C 46.0 0.001500 0.002900 0.003800
D 42.0 0.1291 0.1382 0.1226
E 38.0 1.029 0.9960 0.9513
F 34.0 1.301 1.291 1.404
G 31.0 1.113 1.224 1.145
H 30.0 1.660 1.409 1.491

Protein purified on 11/4/21 and assayed on 11/19/21.
Data uploaded by mcbancks and last updated on 2022-06-27 07:10:33.


Reference Data for WT

slope of A306Q A420 nm
T (°C) 1 2 3
A 50.0 0 0.0005000 0.0007000
B 48.3 0.0009000 0.001100 0.0009000
C 45.7 0.005300 0.006600
D 42.4 0.2606 0.3161 0.3037
E 37.7 0.8638 0.9226 1.137
F 33.6 1.653 1.533 1.388
G 31.3 1.134 1.256 1.339
H 30.0 1.440 1.890 1.644

Protein purified on 11/18/21 and assayed on 11/18/21.
Data uploaded by thuang and last updated on 2022-06-27 07:10:33.


Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.