D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220F

Yield (mg/mL): 3.17

**slope of N220F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.449	1.435	1.401
B	25.00	1.423	1.363	1.37
C	8.33	1.317	1.293	1.252
D	2.78	1.111	1.091	1.131
E	0.93	0.869	0.7441	0.7351
F	0.31	0.3635	0.3309	0.3426
G	0.10	0.1052	0.0984	0.09791
H	0.03	0	0	0

Yield (mg/mL): 3.17 Dilution Factor: 100×

Protein purified on 11/16/21 and assayed on 11/18/21.
Data uploaded by dbvp and last updated on 2023-04-18 09:44:32.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.375E-02	2.090E-02	1.765E-02
B	25.00	2.855E-02	2.355E-02	2.345E-02
C	8.33	2.080E-02	2.210E-02	2.045E-02
D	2.78	1.300E-02	1.205E-02	1.060E-02
E	0.93	6.250E-03	6.050E-03	5.650E-03
F	0.31	2.600E-03	2.650E-03	2.300E-03
G	0.10	1.150E-03	1.000E-03	9.500E-04
H	0.03	5.001E-04		4.500E-04

Yield (mg/mL): 0.25 Dilution Factor: 100×

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by nikchupkin and last updated on 2022-06-27 07:04:03.

**slope of N220F A_{420 nm}**
		T (°C)	1	2	3
	A	30.0	0.001000	0.001000	0.0008000
	B