Raw Data for BglB Variant W325K

Expression Data

Yield (mg/mL): 0.9

Kinetic Assay Data

slope of W325K A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.56E-02	4.05E-02	3.48E-02
	B	25.00	3.62E-02	2.57E-02	2.21E-02
	C	8.33	1.83E-02	2.17E-02	1.83E-02
	D	2.78	1.06E-02		9.45E-03
	E	0.93	4.80E-03	4.50E-03	4.00E-03
	F	0.31	1.80E-03	1.75E-03	1.40E-03
	G	0.10	5.50E-04	6.50E-04	6.00E-04
	H	0.03	3.00E-04	5.00E-04	4.50E-04

Yield (mg/mL): 0.9     Dilution Factor:  10×

Download these data

Protein purified on 11/18/21 and assayed on 11/18/21.
Data uploaded by Sjshinkawa and last updated on 2022-06-27 07:04:03.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of W325K A_{420 nm}

		T (°C)	1	2	3
	A	30.0		8.00E-04	9.00E-04
	B