D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N293V

Expression Data

Yield (mg/mL): 1.24

Kinetic Assay Data

**slope of N293V A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.009000	0.009850	0.01115
B	25.00	0.007650	0.009700	0.01000
C	8.33		0.007450	0.007300
D	2.78	0.003050	0.004050	0.003800
E	0.93		0.001500	0.001500
F	0.31	0.0006001	0.0007500	0.0008000
G	0.10	0	0.0005000	0.0003500
H	0.03	0.0005000	0.0005000	0.0004500

Yield (mm): 1.24 Dilution Factor: 10×

Protein purified on 11/21/21 and assayed on 11/21/21.
Data uploaded by Gabriella and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.375E-02	2.090E-02	1.765E-02
B	25.00	2.855E-02	2.355E-02	2.345E-02
C	8.33	2.080E-02	2.210E-02	2.045E-02
D	2.78	1.300E-02	1.205E-02	1.060E-02
E	0.93	6.250E-03	6.050E-03	5.650E-03
F	0.31	2.600E-03	2.650E-03	2.300E-03
G	0.10	1.150E-03	1.000E-03	9.500E-04
H	0.03	5.001E-04		4.500E-04

Yield (mg/mL): 0.25 Dilution Factor: 100×

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by nikchupkin and last updated on 2022-06-27 07:04:03.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.