Raw Data for BglB Variant D25Q

Expression Data

Yield (mg/mL): 0.638

Kinetic Assay Data

slope of D25Q A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.06535	0.07995	0.07335
	B	25.00	0.0682	0.06675	0.062
	C	8.33	0.04725	0.05315	0.054
	D	2.78	0.03065	0.02925	0.0276
	E	0.93	0.0169	0.01545	0.0141
	F	0.31	0.0061	0.00505	0.0051
	G	0.10		0.0019	0.00185
	H	0.03	0.00095	0.00085	0.00085

Yield (mg/mL): 0.638     Dilution Factor:  100×

Download these data

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by kuocarys and last updated on 2022-06-27 07:04:03.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of D25Q A_{420 nm}

		T (°C)	1	2	3
	A	50.0	0.0004500	0.0006000	0.0005500
	B	48.0	0.0007500	0.0007000	0.0006000
	C	46.0	0.001100	0.001150	0.001050
	D	42.0	0.06225	0.07645	0.05725
	E	38.0	0.3413	0.3452	0.3256
	F	34.0	0.3513	0.3467	0.3569
	G	31.0	0.3537	0.3562	0.3548
	H	30.0	0.3670	0.3775	0.3573

Download these data

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by kuocarys and last updated on 2022-06-27 07:10:33.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.