D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W325K

Expression Data

Yield (mg/mL): 0.48

Kinetic Assay Data

**slope of W325K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.00165	0.0016	0.0013
B	25.00	0.00165	0.0014
C	8.33	0.0011	0.001	0.0008
D	2.78	0.00085		0.00075
E	0.93	0.0005	0.0005
F	0.31	0.00045	0.0004	0.00035
G	0.10	0.0002	0.00025
H	0.03	0.0004	0	0.0002

Yield (mg/mL): 0.48 Dilution Factor: 10×

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by hlau and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3042	0.2949	0.2858
B	25.00	0.2766	0.2821	0.2964
C	8.33	0.1958	0.2027	0.1592
D	2.78	0.1121	0.1206	0.1524
E	0.93	0.04915	0.04830	0.05810
F	0.31	0.01970	0.01795	0.02290
G	0.10	0.006900	0.007300	0.008600
H	0.03	0.003100	0.003000

Yield (mg/mL): 1.546 Dilution Factor: 100×

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by WilliamBenfield and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of W325K A_{420 nm}**
	T (°C)	1	2	3
A	50.0	0.001200	0.001300
B	48.0		0.001400	0.001100
C	46.0		0.005200	0.003700
D	42.0		0.2682	0.2858
E	38.0	0.7912	1.175	1.422
F	34.0	1.520	1.533	1.458
G	31.0	1.662	1.639	1.649
H	30.0	1.761	1.707	1.718

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by WilliamBenfield and last updated on 2022-06-27 07:10:33.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.