Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.25

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.375E-02	2.090E-02	1.765E-02
	B	25.00	2.855E-02	2.355E-02	2.345E-02
	C	8.33	2.080E-02	2.210E-02	2.045E-02
	D	2.78	1.300E-02	1.205E-02	1.060E-02
	E	0.93	6.250E-03	6.050E-03	5.650E-03
	F	0.31	2.600E-03	2.650E-03	2.300E-03
	G	0.10	1.150E-03	1.000E-03	9.500E-04
	H	0.03	5.001E-04		4.500E-04

Yield (mg/mL): 0.25     Dilution Factor:  100×

Download these data

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by nikchupkin and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm}

		T (°C)	1	2	3
	A	50.0	0.0007000	0.0006000	0.0006000
	B	48.0	0.0008000	0.0006000	0.0009000
	C	46.0	0.001400	0.0009000	0.001100
	D	42.0	0.02240	0.01560	0.03040
	E	38.0	0.1276	0.1165	0.1060
	F	34.0	0.1500	0.1544	0.1416
	G	31.0	0.1791	0.1371	0.1639
	H	30.0	0.1869		0.1771

Download these data

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by nikchupkin and last updated on 2022-06-27 07:10:33.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.