Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.546

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.3042	0.2949	0.2858
	B	25.00	0.2766	0.2821	0.2964
	C	8.33	0.1958	0.2027	0.1592
	D	2.78	0.1121	0.1206	0.1524
	E	0.93	0.04915	0.04830	0.05810
	F	0.31	0.01970	0.01795	0.02290
	G	0.10	0.006900	0.007300	0.008600
	H	0.03	0.003100	0.003000

Yield (mg/mL): 1.546     Dilution Factor:  100×

Download these data

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by WilliamBenfield and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm}

		T (°C)	1	2	3
	A	50.0	0.001200	0.001300
	B	48.0		0.001400	0.001100
	C	46.0		0.005200	0.003700
	D	42.0		0.2682	0.2858
	E	38.0	0.7912	1.175	1.422
	F	34.0	1.520	1.533	1.458
	G	31.0	1.662	1.639	1.649
	H	30.0	1.761	1.707	1.718

Download these data

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by WilliamBenfield and last updated on 2022-06-27 07:10:33.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.