Raw Data for BglB Variant N160V

Expression Data

SDS-PAGE
 1

Kinetic Assay Data

slope of N160V A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2503 0.2503 0.2655
B 25.00 0.1789 0.1929 0.1944
C 8.33 0.1072 0.12 0.1206
D 2.78 0.08135 0.09205
E 0.93 0.05365 0.0542 0.0548
F 0.31 0.0243 0.022 0.02205
G 0.10 0.0095 0.0072 0.00805
H 0.03 0.0039 0.00335 0.00355
 1      100×

Protein purified on 10/24/2021 and assayed on 10/26/2021.
Data uploaded by RokhshidR and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.5403 0.5610 0.5446
B 25.00 0.5036 0.5109 0.4649
C 8.33 0.3866 0.3866 0.3604
D 2.78 0.2796 0.2790 0.2534
E 0.93 0.1473 0.1326 0.1282
F 0.31 0.05265 0.04870 0.04480
G 0.10 0.01970 0.01850 0.01385
H 0.03 0.007100 0.007150 0.006650
 2.96      100×

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by kuocarys and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of N160V A420 nm
T (°C) 1 2 3
A 50.0 0.00075 0.00065 0.00065
B 48.3 0.0009 0.001 0.00095
C 45.7 0.0017 0.00165 0.0021
D 42.4 0.07505 0.0762
E 37.7 0.6819 0.7295 0.7441
F 33.6 0.7641 0.8012 0.8178
G 31.3 0.8196 0.8454 0.858
H 30.0 0.8408 0.8791 0.9142

Protein purified on 11/4/2021 and assayed on 11/19/2021.
Data uploaded by RokhshidR and last updated on 2022-06-27 07:10:33.


Reference Data for WT

slope of N160V A420 nm
T (°C) 1 2 3
A 50.0 0.0007000 0.0007500 0.0007000
B 48.0 0.001150 0.001050 0.001300
C 46.0 0.003000 0.003000 0.002950
D 42.0 0.1639 0.1490 0.1773
E 38.0 1.093 1.118 1.098
F 34.0 1.264 1.260 1.276
G 31.0 1.401 1.401 1.378
H 30.0 1.496 1.500 1.497

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by kuocarys and last updated on 2022-06-27 07:10:33.


Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.