Raw Data for BglB Variant S17N

Expression Data

Yield (mg/mL): 0.562

Kinetic Assay Data

slope of S17N A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.00735	0.00705	0.00705
	B	25.00	0.00495	0.0048	0.00435
	C	8.33	0.0042	0.004499	0.00435
	D	2.78	0.00375	0.00345	0.0033
	E	0.93	0.0021	0.0024	0.00225
	F	0.31	0.00165	0.0018	0.00165
	G	0.10	0.00135	0.0012	0.00105
	H	0.03		0	0

Yield (mg/mL): 0.562     Dilution Factor:  100×

Download these data

Protein purified on 1/11/21 and assayed on 8/11/21.
Data uploaded by susanamendiola and last updated on 2022-06-27 07:04:03.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of S17N A_{420 nm}

		T (°C)	1	2	3
	A	30.0	0.0465	0.0405	0.0369
	B	31.0	0.0357	0.0375	0.0318
	C	33.0	0.0111	0.01261	0.0126
	D	38.0	0.0108	0.0105	0.0087
	E	43.0	0.0012	0.003	0.0048
	F	46.0	0	0.0018	0.0012
	G	48.0	0.0015		0.0012
	H	50.0		0.0009	0.0012

Download these data

Protein purified on 1/11/21 and assayed on 8/11/21.
Data uploaded by susanamendiola and last updated on 2022-06-27 07:10:33.

Reference Data for WT

slope of S17N A_{420 nm}

		T (°C)	1	2	3
	A	30.0	0.3798	0.3789	0.3675
	B	31.0	0.3912	0.3699	0.3489
	C	33.0	0.3927	0.3734	0.3687
	D	38.0	0.267	0.2802	0.2775
	E	43.0	0.0054	0.006	0.0048
	F	46.0	0.0012	0.0012	0.0012
	G	48.0	0	0
	H	50.0		0.0012	0.0012

Download these data

Protein purified on 1/11/21 and assayed on 8/11/21.
Data uploaded by CCBestard and last updated on 2022-07-29 12:07:13.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Cristina Baldacci: <cbaldacc@fiu.edu>.