Raw Data for BglB Variant S17N

Expression Data

Kinetic Assay Data

slope of S17N A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.00735	0.00705	0.00705
	B	25.00	0.00495	0.0048	0.00435
	C	8.33	0.0042	0.004499	0.00435
	D	2.78	0.00375	0.00345	0.0033
	E	0.93	0.0021	0.0024	0.00225
	F	0.31	0.00165	0.0018	0.00165
	G	0.10	0.00135	0.0012	0.00105
	H	0.03		0	0

Yield (mg/mL): 0.562     Dilution Factor:  100×

Download these data

Protein purified on 1/11/2021 and assayed on 8/11/2021.
Data uploaded by ajarq008 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

slope of S17N A_{420 nm}

		T (°C)	1	2	3
	A	30.0	3.17E-01	2.90E-01	2.59E-01
	B	31.0	3.26E-01	2.82E-01	2.76E-01
	C	33.0	2.79E-01	2.97E-01	2.96E-01
	D	38.0	9.36E-02	1.04E-01	1.17E-01
	E	43.0	1.80E-03	1.80E-03
	F	46.0	1.50E-03	1.20E-03
	G	48.0	9.00E-04	9.00E-04	9.00E-04
	H	50.0		9.00E-04	9.00E-04

Download these data

Protein purified on 1/11/2021 and assayed on 8/11/2021.
Data uploaded by irest003 and last updated on 2023-09-14 11:09:52.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.