Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.54

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.0813	0.0813	0.07905
	B	25.00	0.0699	0.05835	0.06105
	C	8.33	0.04305	0.03855	0.03825
	D	2.78	0.0177	0.0168	0.01665
	E	0.93	0.0057	0.00525	0.00525
	F	0.31	0.00195		0.00225
	G	0.10	0.00075	0.0009	0.00075
	H	0.03	0		0

Yield (mg/mL): 1.54     Dilution Factor:  100×

Download these data

Protein purified on 1/11/21 and assayed on 8/11/21.
Data uploaded by ssanc207 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm}

		T (°C)	1	2	3
	A	30.0	0.3893	0.3942	0.39
	B	31.0	0.3714	0.3924	0.4113
	C	33.0	0.3834	0.3633	0.381
	D	38.0	0.1467	0.1716	0.1656
	E	43.0	0.0024	0.0021	0.0018
	F	46.0	0.0012	0.0015	0.0015
	G	48.0	0.0012	0.0009	0.0015
	H	50.0	0.0012		0.0015

Download these data

Protein purified on 1/11/21 and assayed on 8/11/21.
Data uploaded by ssanc207 and last updated on 2023-09-11 10:24:31.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.