D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W120N

Expression Data

Yield (mg/mL): 1.214

Kinetic Assay Data

**slope of W120N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.01935	0.01905	0.01755
B	25.00	0.0078	0.00705	0.0078
C	8.33	0.003	0.00285
D	2.78	0.00135	0.0012	0.0012
E	0.93	0.00045	0.00075	0.0006
F	0.31	0		0
G	0.10	0.00075	0.0006	0.0006
H	0.03	0		0

Yield (mg/mL): 1.214 Dilution Factor: 10×

Protein purified on 1/11/21 and assayed on 8/11/21.
Data uploaded by cserr041 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0813	0.0813	0.07905
B	25.00	0.0699	0.05835	0.06105
C	8.33	0.04305	0.03855	0.03825
D	2.78	0.0177	0.0168	0.01665
E	0.93	0.0057	0.00525	0.00525
F	0.31	0.00195		0.00225
G	0.10	0.00075	0.0009	0.00075
H	0.03	0		0

Yield (mg/mL): 1.54 Dilution Factor: 100×

Protein purified on 1/11/21 and assayed on 8/11/21.
Data uploaded by ssanc207 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of W120N A_{420 nm}**
	T (°C)	1	2	3
A	30.0	0.0039	0.003602
B	31.0	0.003002	0.0027	0.0033
C	33.0	0.0027	0.0027	0.0024
D	38.0	0.0018		0.0018
E	43.0	0	0
F	46.0		0
G	48.0		0	0
H	50.0	0	0	0

Protein purified on 1/11/21 and assayed on 8/11/21.
Data uploaded by cserr041 and last updated on 2024-08-08 14:35:13.

Reference Data for WT

**slope of W120N A_{420 nm}**
	T (°C)	1	2	3
A	30.0	0.3893	0.3942	0.39
B	31.0	0.3714	0.3924	0.4113
C	33.0	0.3834	0.3633	0.381
D	38.0	0.1467	0.1716	0.1656
E	43.0	0.0024	0.0021	0.0018
F	46.0	0.0012	0.0015	0.0015
G	48.0	0.0012	0.0009	0.0015
H	50.0	0.0012		0.0015

Protein purified on 1/11/21 and assayed on 8/11/21.
Data uploaded by ssanc207 and last updated on 2023-09-11 10:24:31.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.