D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I91S

Expression Data

Yield (mg/mL): 1.401

Kinetic Assay Data

**slope of I91S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.18E-01	1.04E-01	1.06E-01
B	25.00	8.70E-02	8.30E-02	7.95E-02
C	8.33		9.14E-02	7.38E-02
D	2.78		3.15E-03	5.25E-03
E	0.93	2.70E-03	1.05E-03	1.65E-03
F	0.31		6.00E-04	9.00E-04
G	0.10	6.00E-04		6.00E-04
H	0.03

Yield (mg/mL): 1.401 Dilution Factor: 100×

Protein purified on 29/10/21 and assayed on 5/11/21.
Data uploaded by mayoral and last updated on 2024-05-24 09:39:37.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3629	0.3841	0.3977
B	25.00		0.2968	0.2928
C	8.33	0.2353	0.2502	0.2245
D	2.78	0.08985	0.09615	0.09105
E	0.93	0.0312	0.02805	0.02925
F	0.31	0.0066	0.0066	0.00765
G	0.10	0.00255	0.0024	0.003
H	0.03	0.0009	0.00135	0.00165

Yield (mg/mL): 8.69 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by monke1 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of I91S A_{420 nm}**
	T (°C)	1	2	3
A	30.0	0.3438	0.2796	0.2733
B	31.0	0.2871	0.3045	0.2979
C	33.0	0.3141	0.312	0.2916
D	38.0	0.0879	0.1083	0.1137
E	43.0	0.0021		0.0021
F	46.0	0.0012	0.0012	0.0015
G	48.0
H	50.0		0.0009	0.0012

Protein purified on 29/10/21 and assayed on 05/11/21.
Data uploaded by Yashvi1199 and last updated on 2022-07-29 12:02:12.

Reference Data for WT

**slope of I91S A_{420 nm}**
	T (°C)	1	2	3
A	30.0	0.0228	0.024	0.0219
B	31.0		0.0243
C	33.0	0.0222	0.024	0.0201
D	38.0		0.0135	0.0114
E	43.0	0.0024	0.0021
F	46.0	0.0018	0.0015	0.0012
G	48.0	0.0015	0.0012	0.0015
H	50.0	0		0

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by danielamorey and last updated on 2022-07-29 12:18:06.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.