D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I91R

Expression Data

Yield (mg/mL): 0.8955

Kinetic Assay Data

**slope of I91R A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0498	0.048	0.05085
B	25.00	0.0369	0.0372	0.03705
C	8.33	0.039	0.03405	0.0453
D	2.78	0.0042	0.00315	0.0039
E	0.93	0.00165	0.0012	0.00165
F	0.31	0.00075	0.00075	0.00075
G	0.10	0.00075	0.00075	0.0006002
H	0.03	0.00105		0.00075

Yield (mg/mL): 0.8955 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by jprad025 and last updated on 2023-04-18 10:30:38.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2814	0.2931	0.2946
B	25.00		0.2127	0.2292
C	8.33	0.101	0.1119	0.1399
D	2.78	0.0384	0.04516	0.06135
E	0.93	0.009752	0.01155
F	0.31	0.0033	0.00315
G	0.10	0.00105	0.00105
H	0.03	0.00075	0.0009	0.00105

Yield (mg/mL): 4.691 Dilution Factor: 100×

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by msimon01 and last updated on 2023-09-11 17:36:42.

Thermostability Assay Data

**slope of I91R A_{420 nm}**
	T (°C)	1	2	3
A	30.0		0.2433
B	31.0	0.2379	0.2223	0.195
C	33.0	0.231	0.2184	0.2091
D	38.0	0.0567	0.0525	0.0453
E	43.0		0.0015	0.0018
F	46.0	0	0
G	48.0	0.0027	0	0.0015
H	50.0		0.0015	0.0015

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by jprad025 and last updated on 2023-04-18 10:34:03.

Reference Data for WT

**slope of I91R A_{420 nm}**
	T (°C)	1	2	3
A	30.0	1.587	1.559	1.571
B	31.0	1.354	1.332	1.491
C	33.0	1.729	1.428	1.373
D	38.0	0.5814	0.5826	0.5559
E	43.0		0.0057	0.0054
F	46.0	0.0015	0.0021	0.0024
G	48.0	0.0048		0.0021
H	50.0	0.0018	0.0021

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by msimon01 and last updated on 2023-09-11 17:37:51.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.