D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220C

Expression Data

Yield (mg/mL): 1.045

Kinetic Assay Data

**slope of N220C A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.04245	0.04155	0.04095
B	25.00	0.04155	0.04185	0.0405
C	8.33	0.0333	0.0333	0.03345
D	2.78	0.01515	0.01545	0.0132
E	0.93	0.0069	0.0054	0.0045
F	0.31	0.00195	0.00195	0.0018
G	0.10	0.0021	0.00165	0.0015
H	0.03	0.0006002	0.00075	0.0009

Yield (mg/mL): 1.045 Dilution Factor: 100×

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by cgonz615 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3639	0.3318	0.3155
B	25.00	0.2994	0.2659	0.2694
C	8.33		0.1747	0.177
D	2.78	0.0708	0.0672	0.06525
E	0.93	0.0216	0.0204	0.01935
F	0.31	0.00525	0.00525	0.00495
G	0.10	0.00195	0.0018	0.0018
H	0.03	0.0012	0.00105	0.0012

Yield (mg/mL): 4.362 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by jsalg031 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of N220C A_{420 nm}**
	T (°C)	1	2	3
A	30.0
B	31.0	0.2946	0.2829	0.2799
C	33.0	0.2652	0.2589	0.2463
D	38.0	0.087	0.0708	0.0735
E	43.0	0.0018	0.0015	0.0018
F	46.0	0	0	0
G	48.0	0	0	0
H	50.0	0	0	0

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by cgonz615 and last updated on 2024-08-08 18:46:27.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.