D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220C

Expression Data

Yield (mg/mL): 0.7828

Kinetic Assay Data

**slope of N220C A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.03225		0.0279
B	25.00	0.0258	0.02595	0.0252
C	8.33	0.02085	0.0207	0.02115
D	2.78	0.01035	0.00945	0.00945
E	0.93	0.0027	0.00315	0.00345
F	0.31		0.00105	0.00105
G	0.10	0.0009	0.0009	0.0009
H	0.03	0.00075	0.00075

Yield (mg/mL): 0.7828 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by slash817 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3639	0.3318	0.3155
B	25.00	0.2994	0.2659	0.2694
C	8.33		0.1747	0.177
D	2.78	0.0708	0.0672	0.06525
E	0.93	0.0216	0.0204	0.01935
F	0.31	0.00525	0.00525	0.00495
G	0.10	0.00195	0.0018	0.0018
H	0.03	0.0012	0.00105	0.0012

Yield (mg/mL): 4.362 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by jsalg031 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of N220C A_{420 nm}**
	T (°C)	1	2	3
A	30.0	0.1992	0.1761	0.1614
B	31.2	0.2122	0.2095	0.1617
C	33.0	0.2169	0.2049	0.1951
D	37.9	0.0654	0.0669	0.07594
E	43.4	0.001501		0.0018
F	46.0		0.0015	0.0012
G	48.2	0.0012		0.0021
H	50.0		0.0012	0.0009

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by slash817 and last updated on 2022-07-29 12:00:09.

Reference Data for WT

**slope of N220C A_{420 nm}**
	T (°C)	1	2	3
A	30.0	2.13	1.859	1.702
B	31.0	2.044	1.974	1.91
C	33.0	1.438	1.428
D	38.0	0.9098	1.061	0.9905
E	43.0	0.01051	0.01111	0.0102
F	46.0		0.0015	0.0012
G	48.0		0.0015	0.0012
H	50.0	0.0015	0.0012	0.0015

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by jsalg031 and last updated on 2022-07-29 12:13:52.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.