D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220L

Expression Data

Yield (mg/mL): 4.511

Kinetic Assay Data

**slope of N220L A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.5824	0.5674	0.5759
B	25.00	0.5016	0.5272	0.5239
C	8.33	0.4386	0.4659	0.4547
D	2.78	0.2694	0.226	0.2187
E	0.93	0.1107	0.1067	0.1181
F	0.31	0.03165	0.02881	0.03211
G	0.10	0.0087	0.008252	0.0081
H	0.03	0.0024	0.002401	0.00255

Yield (mg/mL): 4.511 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by klope191 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3639	0.3318	0.3155
B	25.00	0.2994	0.2659	0.2694
C	8.33		0.1747	0.177
D	2.78	0.0708	0.0672	0.06525
E	0.93	0.0216	0.0204	0.01935
F	0.31	0.00525	0.00525	0.00495
G	0.10	0.00195	0.0018	0.0018
H	0.03	0.0012	0.00105	0.0012

Yield (mg/mL): 4.362 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by jsalg031 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of N220L A_{420 nm}**
	T (°C)	1	2	3
A	30.0		2.989	2.772
B	31.0			2.686
C	33.0
D	38.0	1.476	1.563
E	43.0	0.1239	0.1053	0.0747
F	46.0	0.0123	0.012	0.0129
G	48.0	0.0024	0.0024
H	50.0	0.0018	0.0018

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by klope191 and last updated on 2023-02-01 12:53:10.

Reference Data for WT

**slope of N220L A_{420 nm}**
	T (°C)	1	2	3
A	30.0	2.13	1.859	1.702
B	31.0	2.044	1.974	1.91
C	33.0	1.438	1.428
D	38.0	0.9098	1.061	0.9905
E	43.0	0.01051	0.01111	0.0102
F	46.0		0.0015	0.0012
G	48.0		0.0015	0.0012
H	50.0	0.0015	0.0012	0.0015

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by jsalg031 and last updated on 2022-07-29 12:13:52.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.