D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220S

Expression Data

Yield (mg/mL): 1.282

Kinetic Assay Data

**slope of N220S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		0.01935	0.02295
B	25.00		0.0114	0.01155
C	8.33		0.00435	0.004501
D	2.78		0.00165	0.00195
E	0.93		0.00105	0.00105
F	0.31		0.00075
G	0.10	0.0009	0.00105	0.0006
H	0.03

Yield (mg/mL): 1.282 Dilution Factor: 10×

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by RachelSanchez and last updated on 2024-05-09 11:15:42.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3141	0.2675	0.2739
B	25.00	0.2138	0.1932	0.2226
C	8.33	0.1445	0.1437	0.1467
D	2.78	0.07215	0.0687	0.0645
E	0.93	0.0222	0.02205	0.0198
F	0.31	0.0066	0.00645	0.006
G	0.10	0.00225	0.0021	0.001951
H	0.03	0.0012	0.0012	0.0015

Yield (mg/mL): 3.174 Dilution Factor: 100×

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by danielamorey and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of N220S A_{420 nm}**
	T (°C)	1	2	3
A	30.0
B	31.0	0.021	0.024	0.0213
C	33.0	0.0192	0.0204	0.0165
D	38.0	0.0138	0.0123	0.0138
E	43.0	0.003	0.003
F	46.0	0.0018	0.0015	0.0012
G	48.0	0.0015	0.0012	0.0021
H	50.0	0.0015	0.0015

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by RachelSanchez and last updated on 2022-07-28 18:12:24.

Reference Data for WT

**slope of N220S A_{420 nm}**
	T (°C)	1	2	3
A	30.0	0.0228	0.024	0.0219
B	31.0		0.0243
C	33.0	0.0222	0.024	0.0201
D	38.0		0.0135	0.0114
E	43.0	0.0024	0.0021
F	46.0	0.0018	0.0015	0.0012
G	48.0	0.0015	0.0012	0.0015
H	50.0	0		0

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by danielamorey and last updated on 2022-07-29 12:18:06.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.