D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220F

Expression Data

Yield (mg/mL): 2.531

Kinetic Assay Data

**slope of N220F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.09705	0.0963	0.1013
B	25.00	0.0963	0.0924	0.09825
C	8.33	0.08265	0.08668	0.0867
D	2.78	0.06945	0.0672	0.0672
E	0.93	0.0393	0.04035	0.0351
F	0.31	0.01485	0.01755	0.01425
G	0.10	0.00435		0.0039
H	0.03	0.00315	0.00225	0.0015

Yield (mg/mL): 2.531 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by lijooni and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3639	0.3318	0.3155
B	25.00	0.2994	0.2659	0.2694
C	8.33		0.1747	0.177
D	2.78	0.0708	0.0672	0.06525
E	0.93	0.0216	0.0204	0.01935
F	0.31	0.00525	0.00525	0.00495
G	0.10	0.00195	0.0018	0.0018
H	0.03	0.0012	0.00105	0.0012

Yield (mg/mL): 4.362 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by jsalg031 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of N220F A_{420 nm}**
	T (°C)	1	2	3
A	30.0
B	31.0	0.7134	0.7438	0.6741
C	33.0	0.6264	0.6927	0.6951
D	38.0	0.2811	0.3099
E	43.0	0.0102	0.0084
F	46.0	0.0018	0.0018	0.0015
G	48.0	0.0012	0.0012	0.0012
H	50.0		0.0015	0.0018

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by lijooni and last updated on 2022-07-29 12:23:57.

Reference Data for WT

**slope of N220F A_{420 nm}**
	T (°C)	1	2	3
A	30.0	2.13	1.859	1.702
B	31.0	2.044	1.974	1.91
C	33.0	1.438	1.428
D	38.0	0.9098	1.061	0.9905
E	43.0	0.01051	0.01111	0.0102
F	46.0		0.0015	0.0012
G	48.0		0.0015	0.0012
H	50.0	0.0015	0.0012	0.0015

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by jsalg031 and last updated on 2022-07-29 12:13:52.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.