Raw Data for BglB Variant N220F

Expression Data

SDS-PAGE
 1.176

Kinetic Assay Data

slope of N220F A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.03345 0.03135
B 25.00 0.02835 0.02895 0.02955
C 8.33 0.0306 0.0291 0.03045
D 2.78 0.01965 0.01935 0.02295
E 0.93 0.0123 0.01035 0.01155
F 0.31 0.00405 0.0045 0.0042
G 0.10 0.00165 0.0018 0.0018
H 0.03 0.0007502 0.0009 0.0009
 1.176      100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by jsalg031 and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3639 0.3318 0.3155
B 25.00 0.2994 0.2659 0.2694
C 8.33 0.1747 0.177
D 2.78 0.0708 0.0672 0.06525
E 0.93 0.0216 0.0204 0.01935
F 0.31 0.00525 0.00525 0.00495
G 0.10 0.00195 0.0018 0.0018
H 0.03 0.0012 0.00105 0.0012
 4.362      100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by jsalg031 and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of N220F A420 nm
T (°C) 1 2 3
A 30.0 0.2584 0.2233 0.168
B 31.0 0.3368 0.2785 0.2947
C 33.0 0.2632 0.2211 0.277
D 38.0 0.08944 0.09815
E 43.0 0.003002 0.0027 0.0027
F 46.0 0.0015 0.0018 0.0018
G 48.0 0.0015 0.0015 0.0015
H 50.0 0.001799 0.0012 0.0015

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by jsalg031 and last updated on 2023-03-29 11:31:21.


Reference Data for WT

slope of N220F A420 nm
T (°C) 1 2 3
A 30.0 2.13 1.859 1.702
B 31.0 2.044 1.974 1.91
C 33.0 1.438 1.428
D 38.0 0.9098 1.061 0.9905
E 43.0 0.01051 0.01111 0.0102
F 46.0 0.0015 0.0012
G 48.0 0.0015 0.0012
H 50.0 0.0015 0.0012 0.0015

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by jsalg031 and last updated on 2022-07-29 12:13:52.


Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.