D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220L

Expression Data

Yield (mg/mL): 4.523

Kinetic Assay Data

**slope of N220L A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00	6.15E-03	6.45E-03
C	8.33	2.85E-03	2.85E-03
D	2.78	1.65E-03	1.50E-03	1.80E-03
E	0.93		9.00E-04	7.50E-04
F	0.31	9.00E-04	9.00E-04
G	0.10
H	0.03

Yield (mg/mL): 4.523 Dilution Factor: 100×

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by JohnathanG and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3141	0.2675	0.2739
B	25.00	0.2138	0.1932	0.2226
C	8.33	0.1445	0.1437	0.1467
D	2.78	0.07215	0.0687	0.0645
E	0.93	0.0222	0.02205	0.0198
F	0.31	0.0066	0.00645	0.006
G	0.10	0.00225	0.0021	0.001951
H	0.03	0.0012	0.0012	0.0015

Yield (mg/mL): 3.174 Dilution Factor: 100×

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by danielamorey and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

**slope of N220L A_{420 nm}**
	T (°C)	1	2	3
A	30.0	0.6504	0.1072	0.4890
B	31.0	0.8190		0.9774
C	33.0	0.4083		0.5136
D	38.0
E	43.0	0.02910	0.02670	0.02460
F	46.0	0.003900	0.003900
G	48.0	0.001800		0.001800
H	50.0	0.001500	0.001500	0.001500

Protein purified on 10/29/2021 and assayed on 11/5/2021.
Data uploaded by JohnathanG and last updated on 2022-07-29 12:10:54.

Reference Data for WT

**slope of N220L A_{420 nm}**
	T (°C)	1	2	3
A	30.0	0.0228	0.024	0.0219
B	31.0		0.0243
C	33.0	0.0222	0.024	0.0201
D	38.0		0.0135	0.0114
E	43.0	0.0024	0.0021
F	46.0	0.0018	0.0015	0.0012
G	48.0	0.0015	0.0012	0.0015
H	50.0	0		0

Protein purified on 10/29/21 and assayed on 11/5/21.
Data uploaded by danielamorey and last updated on 2022-07-29 12:18:06.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.