D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Y295N

Expression Data

Yield (mg/mL): 1.589

Kinetic Assay Data

**slope of Y295N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.04380	0.06005	0.04805
B	25.00	0.03830	0.04170	0.04875
C	8.33	0.01870	0.01825	0.01470
D	2.78	0.007850	0.006350	0.003700
E	0.93	0.003000	0.002500	0.001650
F	0.31	0.001300	0.001250
G	0.10	0.0004500	0.0005000	0.0003500
H	0.03	0.0003500	0	0.0002000

Yield (mg/mL): 1.589 Dilution Factor: 100×

Protein purified on 10/24/2021 and assayed on 10/26/2021.
Data uploaded by bmagana1 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3435	0.3344	0.3088
B	25.00	0.2549	0.2855	0.2801
C	8.33	0.2361	0.2431	0.2529
D	2.78	0.1373	0.1505	0.1205
E	0.93	0.05565	0.07400	0.07160
F	0.31		0.02880	0.02640
G	0.10		0.01110	0.01120
H	0.03	0.002950	0.004350	0.003850

Yield (mg/mL): 3.334 Dilution Factor: 100×

Protein purified on 4/10/2022 and assayed on 4/11/2022.
Data uploaded by bmagana1 and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.