Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.04E-01	2.11E-01	2.08E-01
	B	25.00	1.72E-01	1.77E-01	1.81E-01
	C	8.33	1.29E-01	1.37E-01	1.49E-01
	D	2.78	6.70E-02	7.05E-02	7.23E-02
	E	0.93	4.91E-02	4.93E-02	4.58E-02
	F	0.31	2.66E-02	2.59E-02	2.89E-02
	G	0.10	1.51E-02	1.33E-02	1.58E-02
	H	0.03	0	1.00E-04	1.50E-04

Yield (mg/mL): 2.076     Dilution Factor:  100×

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Protein purified on 4/2/2025 and assayed on 4/9/2025.
Data uploaded by srumjahn and last updated on 2025-04-22 11:51:43.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	2.00E-04	2.00E-04	2.50E-04
	B	48.3	2.50E-04		3.00E-04
	C	45.7	5.50E-04	6.00E-04	5.50E-04
	D	42.4	2.25E-02	2.38E-02	2.68E-02
	E	37.7	1.01E-01	1.04E-01	1.00E-01
	F	33.6	9.69E-02	1.03E-01	1.09E-01
	G	31.3	1.11E-01	1.13E-01	1.10E-01
	H	30.0	1.01E-01	1.09E-01	1.20E-01

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Protein purified on 4/2/2025 and assayed on 4/16/2025.
Data uploaded by srumjahn and last updated on 2025-04-22 11:57:41.

Citations

The unpublished data above were collected by students from Truckee Meadows Community College. For inquiries, please contact Sharif M Rumjahn: <srumjahn@tmcc.edu>.