Raw Data for BglB Variant W399H

Expression Data

SDS-PAGE
 0.172

Kinetic Assay Data

slope of W399H A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 7.50E-03 7.50E-03
B 25.00 3.00E-03 5.00E-04 4.00E-03
C 8.33 2.00E-03 2.00E-03
D 2.78 5.00E-04 5.00E-04
E 0.93 5.00E-04 5.00E-04
F 0.31 5.00E-04 5.00E-04
G 0.10
H 0.03
 0.172      10×

Protein purified on 2/27/25 and assayed on 3/27/25.
Data uploaded by JaysonWright and last updated on 2025-04-22 08:37:39.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 5.15E-02 4.70E-02 4.60E-02
B 25.00 3.30E-02 3.40E-02 3.20E-02
C 8.33 2.45E-02 2.25E-02
D 2.78 1.35E-02 1.20E-02 1.30E-02
E 0.93 5.00E-03 5.00E-03
F 0.31 2.50E-03 1.50E-03 3.50E-03
G 0.10 2.50E-03 1.00E-03 5.00E-04
H 0.03 1.00E-03 1.00E-03
 0.204      100×

Protein purified on 2/27/25 and assayed on 3/27/25.
Data uploaded by JaysonWright and last updated on 2025-04-22 08:42:39.


Thermostability Assay Data

slope of W399H A420 nm (min−1)
T (°C) 1 2 3
A 50.0 5.00E-04 5.00E-04
B 48.3 0.00E+00
C 45.7 0.00E+00
D 42.4 1.50E-03 1.50E-03
E 37.7 1.50E-03 1.50E-03
F 33.6 1.00E-03 1.00E-03
G 31.3 2.00E-03 2.00E-03
H 30.0 1.50E-03 1.50E-03

Protein purified on 2/27/25 and assayed on 4/3/25.
Data uploaded by JaysonWright and last updated on 2025-04-22 08:33:52.


Reference Data for WT

slope of W399H A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00E+00 0.00E+00
B 48.3 5.00E-04 5.00E-04 5.00E-04
C 45.7 5.00E-04 5.00E-04 5.00E-04
D 42.4 1.00E-02 6.50E-03 8.50E-03
E 37.7 2.85E-02 2.90E-02 2.40E-02
F 33.6 3.05E-02 3.70E-02
G 31.3 4.00E-02
H 30.0 4.25E-02 3.40E-02 3.05E-02

Protein purified on 2/27/25 and assayed on 4/3/25.
Data uploaded by JaysonWright and last updated on 2025-04-22 08:44:00.


Design Data

5.494

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.