Raw Data for BglB Variant E312H

Expression Data

Yield (mg/mL): 0.367

Kinetic Assay Data

slope of E312H A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	7.70E-02	8.15E-02	7.65E-02
	B	25.00	5.65E-02	5.30E-02	5.35E-02
	C	8.33	3.65E-02	3.55E-02	3.65E-02
	D	2.78	1.85E-02	2.30E-02	2.20E-02
	E	0.93	7.00E-03	7.50E-03
	F	0.31	3.50E-03	3.50E-03	4.00E-03
	G	0.10	1.00E-03	1.00E-03
	H	0.03

Yield (mg/mL): 0.269     Dilution Factor:  100×

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Protein purified on 2/28/25 and assayed on 3/28/25.
Data uploaded by NolynMjema121 and last updated on 2025-04-17 13:30:44.

Thermostability Assay Data

slope of E312H A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0
	B	48.3		5.00E-04	5.00E-04
	C	45.7	0.00E+00		0.00E+00
	D	42.4	5.50E-03	6.50E-03	6.00E-03
	E	37.7	4.85E-02	4.20E-02	4.15E-02
	F	33.6	5.70E-02	6.25E-02	6.10E-02
	G	31.3	6.05E-02	6.05E-02	6.05E-02
	H	30.0	6.35E-02	6.20E-02	6.10E-02

Download these data

Protein purified on 2/28/25 and assayed on 4/4/25.
Data uploaded by NolynMjema121 and last updated on 2025-04-17 13:51:54.

Design Data

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.