Raw Data for BglB Variant A226E

Expression Data

SDS-PAGE
 0.228

Kinetic Assay Data

slope of A226E A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.05E-01 8.40E-02 6.80E-02
B 25.00 5.25E-02 5.30E-02 5.00E-02
C 8.33 3.10E-02 3.40E-02 3.35E-02
D 2.78 2.00E-02 1.65E-02 1.75E-02
E 0.93 6.50E-03 6.50E-03 7.00E-03
F 0.31 2.00E-03 2.00E-03 2.50E-03
G 0.10 1.00E-03 1.00E-03
H 0.03 0 0.00E+00
 0.168      100×

Protein purified on 2/27/25 and assayed on 3/27/25.
Data uploaded by AnnaOlszowka and last updated on 2025-04-17 13:30:49.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.35E-02 2.05E-02 1.85E-02
B 25.00 1.55E-02 1.55E-02 1.40E-02
C 8.33 1.10E-02 9.50E-03 8.50E-03
D 2.78 5.00E-03 5.00E-03 4.50E-03
E 0.93 3.00E-03 3.00E-03
F 0.31 5.00E-04 5.00E-04
G 0.10 5.00E-04 5.00E-04
H 0.03 0.00E+00 0.00E+00 0.00E+00
 0.107      100×

Protein purified on 2/27/25 and assayed on 3/27/25.
Data uploaded by CullenMatthews and last updated on 2025-04-17 13:51:32.


Thermostability Assay Data

slope of A226E A420 nm (min−1)
T (°C) 1 2 3
A 50.0 5.00E-04 5.00E-04 5.00E-04
B 48.3 5.00E-04 5.00E-04 5.00E-04
C 45.7 1.00E-03 5.00E-04 0.00E+00
D 42.4 4.00E-03 4.00E-03 3.50E-03
E 37.7 2.35E-02 2.45E-02 2.30E-02
F 33.6 3.95E-02 3.95E-02
G 31.3 4.40E-02 4.20E-02
H 30.0 4.60E-02 4.45E-02

Protein purified on 2/27/25 and assayed on 4/3/25.
Data uploaded by AnnaOlszowka and last updated on 2025-04-17 13:41:30.


Reference Data for WT

slope of A226E A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0005 0.0005
B 48.3 0.0005 0.0005 0.0005
C 45.7 0.0005 0.0005
D 42.4 0.002 0.002 0.002
E 37.7 0.0045 0.004 0.005
F 33.6 0.005 0.0055 0.006
G 31.3 0.005 0.006 0.005
H 30.0 0.005

Protein purified on 06/03/2025 and assayed on 03/04/2025.
Data uploaded by nickyw96 and last updated on 2025-04-17 14:19:30.


Design Data

4.801

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.