Raw Data for BglB Variant E326T

Expression Data

SDS-PAGE
 0.0755

Kinetic Assay Data

slope of E326T A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 9.00E-02 8.30E-02 8.70E-02
B 25.00 7.20E-02 7.25E-02 6.90E-02
C 8.33 4.75E-02 4.80E-02 4.75E-02
D 2.78 2.80E-02 2.90E-02 2.60E-02
E 0.93 1.05E-02 1.05E-02 1.10E-02
F 0.31 4.50E-03 4.50E-03 4.00E-03
G 0.10 1.50E-03 1.50E-03
H 0.03 5.00E-04 5.00E-04
 0.0555368      10×

Protein purified on 2/27/25 and assayed on 3/27/25.
Data uploaded by PriyaGill and last updated on 2025-04-17 13:32:09.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.40E-02 1.90E-02 1.75E-02
B 25.00 1.65E-02 1.45E-02 1.35E-02
C 8.33 1.05E-02 1.10E-02 9.50E-03
D 2.78 6.50E-03 6.50E-03 6.50E-03
E 0.93 3.00E-03 3.00E-03 3.50E-03
F 0.31 1.50E-03 1.50E-03 1.50E-03
G 0.10 7.40E-17 7.40E-17
H 0.03 0.00E+00 0.00E+00 0.00E+00
 0.113824      100×

Protein purified on 2/27/25 and assayed on 3/27/25.
Data uploaded by PriyaGill and last updated on 2025-04-17 13:35:53.


Thermostability Assay Data

Data missing or deleted from the database.


Reference Data for WT

slope of E326T A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 7.00E-04
B 48.3 4.00E-04 5.00E-04 6.00E-04
C 45.7 6.00E-04 9.00E-04 7.00E-04
D 42.4 4.12E-02 3.96E-02 4.71E-02
E 37.7 2.14E-01 1.91E-01 2.10E-01
F 33.6 2.47E-01 2.35E-01 2.36E-01
G 31.3 2.18E-01 2.14E-01 2.05E-01
H 30.0 2.62E-01 2.56E-01 2.62E-01

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 20:10:03.


Design Data

1088.7

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.