Raw Data for BglB Variant T116A
Expression Data
0.175
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 1.35E-02 | 1.45E-02 | |||
| B | 25.00 | 7.50E-03 | 9.00E-03 | 7.00E-03 | ||
| C | 8.33 | 5.50E-03 | 5.00E-03 | |||
| D | 2.78 | 4.00E-03 | 3.00E-03 | 3.50E-03 | ||
| E | 0.93 | 5.00E-03 | 3.00E-03 | 1.50E-03 | ||
| F | 0.31 | 3.00E-03 | 4.00E-03 | 4.00E-03 | ||
| G | 0.10 | 1.50E-03 | 1.50E-03 | |||
| H | 0.03 | 5.00E-04 | 1.00E-03 |
Protein purified on 2/27/2025 and assayed on 3/27/2025.
Data uploaded by radianasimeonova and last updated on 2025-04-17 13:19:30.
Thermostability Assay Data
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 30.0 | 6.35E-02 | 6.25E-02 | 6.25E-02 | ||
| B | 31.3 | 6.30E-02 | 6.30E-02 | 6.65E-02 | ||
| C | 33.8 | 6.20E-02 | 6.10E-02 | 6.05E-02 | ||
| D | 37.4 | 4.10E-02 | 4.00E-02 | 4.15E-02 | ||
| E | 42.2 | 2.00E-03 | 2.00E-03 | 2.00E-03 | ||
| F | 45.9 | 0.00E+00 | 0.00E+00 | 0.00E+00 | ||
| G | 48.4 | 5.00E-04 | 5.00E-04 | 5.00E-04 | ||
| H | 50.0 | 0.00E+00 | 0.00E+00 |
Reference Data for WT
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 30.0 | 1.40E-02 | 1.45E-02 | 1.60E-02 | ||
| B | 31.3 | 1.35E-02 | 1.45E-02 | 1.40E-02 | ||
| C | 33.6 | 1.40E-02 | 1.50E-02 | 1.50E-02 | ||
| D | 37.7 | 1.30E-02 | 1.30E-02 | 1.20E-02 | ||
| E | 42.4 | 0.0045 | 0.0045 | |||
| F | 45.7 | 0.0005 | 0.0005 | 0.0005 | ||
| G | 48.3 | 0 | 0.0005 | 0.001 | ||
| H | 50.0 | 1.48e-16 | 1.48e-16 |
Protein purified on 2/27/2025 and assayed on 4/3/2025.
Data uploaded by KaylaYale and last updated on 2025-04-17 13:27:05.
Design Data
Citations
The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.