Raw Data for BglB Variant N304W

Expression Data

SDS-PAGE
 not reported

Kinetic Assay Data

slope of N304W A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.70E-02 2.60E-02 2.65E-02
B 25.00 2.15E-02 2.10E-02 2.10E-02
C 8.33 1.55E-02 1.40E-02 1.55E-02
D 2.78 9.50E-03 8.50E-03 9.50E-03
E 0.93 4.00E-03 3.50E-03 4.50E-03
F 0.31 2.00E-03 1.50E-03 1.50E-03
G 0.10 1.00E-03 1.00E-03 1.00E-03
H 0.03 0.00E+00 0.00E+00
 0.15      100×

Protein purified on 2/27/25 and assayed on 3/27/25.
Data uploaded by scobioma and last updated on 2025-04-15 18:11:54.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.0305 0.0315 0.0315
B 25.00 0.028 0.031 0.025
C 8.33 0.017 0.017 0.017
D 2.78 0.01 0.01 0.0105
E 0.93 0.0045 0.0045 0.004
F 0.31 0.002 0.002 0.002
G 0.10 0.0005 0.0005
H 0.03 7.401E-17 7.401E-17
 0.094      100×

Protein purified on 2/27/25 and assayed on 3/27/25.
Data uploaded by scobioma and last updated on 2025-04-15 17:04:37.


Thermostability Assay Data

slope of N304W A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 1.48E-16 0
C 45.7 0 0
D 42.4 0.0005 0.0005
E 37.7 0.0055 0.005
F 33.6 0.009 0.0085 0.008
G 31.3 0.0095 0.01 0.0095
H 30.0 0.0095 0.009 0.009

Protein purified on 2/27/25 and assayed on 4/3/25.
Data uploaded by scobioma and last updated on 2025-04-15 18:12:35.


Reference Data for WT

slope of N304W A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 0.0005 0.0005
C 45.7 0.0005 0.0005
D 42.4 0.0155 0.0145 0.0135
E 37.7 0.037 0.033 0.034
F 33.6 0.046 0.0455 0.0415
G 31.3 0.0515 0.053 0.042
H 30.0 0.0525 0.0505 0.042

Protein purified on 2/27/25 and assayed on 4/3/25.
Data uploaded by scobioma and last updated on 2025-04-15 17:14:25.


Design Data

5.601

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.