Raw Data for BglB Variant N157Q

Expression Data

Yield (mg/mL): not reported

Thermostability Assay Data

slope of N157Q A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0.00E+00	0.00E+00
	B	48.3	5.00E-04	5.00E-04	5.00E-04
	C	45.7	5.50E-03	5.50E-03	5.00E-03
	D	42.4	6.35E-02	7.00E-02	7.15E-02
	E	37.7	9.30E-02	1.02E-01	1.08E-01
	F	33.6	9.75E-02	1.04E-01	1.09E-01
	G	31.3	9.55E-02	1.05E-01	1.06E-01
	H	30.0	1.05E-01	1.21E-01	1.22E-01

Download these data

Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by angelinaguirgis and last updated on 2025-04-15 14:18:47.

Reference Data for WT

slope of N157Q A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	5.00E-04	5.00E-04	5.00E-04
	B	48.3	5.00E-04		5.00E-04
	C	45.7	4.50E-03	4.00E-03	3.50E-03
	D	42.4	1.62E-01	1.57E-01	1.75E-01
	E	37.7	3.41E-01	3.54E-01	3.40E-01
	F	33.6	3.83E-01	3.85E-01	3.86E-01
	G	31.3	3.55E-01	3.45E-01	3.56E-01
	H	30.0	3.72E-01	3.75E-01	3.70E-01

Download these data

Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by angelinaguirgis and last updated on 2025-04-15 14:34:43.

Design Data

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.