Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.2875

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00		2.02E-01	2.23E-01
	B	25.00	3.00E-02	1.99E-01	1.40E-01
	C	8.33		1.49E-01	1.77E-01
	D	2.78	1.40E-02	9.10E-02	5.80E-02
	E	0.93		4.40E-02	4.90E-02
	F	0.31	3.00E-03	1.70E-02	1.20E-02
	G	0.10		7.00E-03	6.50E-03
	H	0.03	0.00E+00	5.00E-04	1.00E-03

Yield (mg/mL): 0.2875     Dilution Factor:  10×

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Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by tranisabella and last updated on 2025-04-15 14:32:02.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	30.0	5.70E-02	5.70E-02	5.80E-02
	B	31.3	5.80E-02	5.65E-02	5.55E-02
	C	33.6	5.80E-02	6.20E-02	6.00E-02
	D	37.7	4.65E-02	4.50E-02	4.70E-02
	E	42.4	1.80E-02	1.65E-02	1.75E-02
	F	45.7		5.00E-04	5.00E-04
	G	48.3		5.00E-04	5.00E-04
	H	50.0		7.40E-17	0.00E+00

Download these data

Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by tranisabella and last updated on 2025-04-15 14:09:23.

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.