Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.1803

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	8.60E-02	7.85E-02	7.55E-02
	B	25.00	6.40E-02	6.60E-02	6.15E-02
	C	8.33	4.70E-02	4.15E-02	4.80E-02
	D	2.78	2.90E-02	2.90E-02	2.85E-02
	E	0.93	1.35E-02	1.30E-02	1.30E-02
	F	0.31	5.50E-03	5.00E-03	5.00E-03
	G	0.10	2.50E-03	2.00E-03	2.00E-03
	H	0.03	7.40E-17		7.40E-17

Yield (mg/mL): 0.1803     Dilution Factor:  10×

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Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by ushuunamarra and last updated on 2025-04-15 14:39:54.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.00E+00	0.00E+00
	B	48.3	5.00E-04	5.00E-04	5.00E-04
	C	45.7	1.00E-03	1.00E-03
	D	42.4	1.90E-02	1.65E-02	1.90E-02
	E	37.7	4.30E-02	3.90E-02	3.80E-02
	F	33.6	4.70E-02	4.65E-02	4.40E-02
	G	31.3	4.45E-02	4.45E-02	4.50E-02
	H	30.0	4.55E-02	4.60E-02	4.10E-02

Download these data

Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by ushuunamarra and last updated on 2025-04-15 14:23:50.

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.