Raw Data for BglB Variant E340L

Expression Data

SDS-PAGE
 0.0565

Kinetic Assay Data

slope of E340L A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.50E-03 4.50E-03 4.50E-03
B 25.00 4.00E-03 4.00E-03 3.50E-03
C 8.33 3.00E-03 2.50E-03 2.50E-03
D 2.78 2.00E-03 2.50E-03 2.00E-03
E 0.93 1.00E-03 1.00E-03
F 0.31 5.00E-04 1.50E-03 2.00E-03
G 0.10 5.00E-04 0.00E+00 1.00E-03
H 0.03 0.00E+00 0.00E+00
 0.042      10×

Protein purified on 2/25/2025 and assayed on 3/25/2025.
Data uploaded by anandsrinivasan and last updated on 2025-04-15 14:28:00.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.02E-01 2.23E-01
B 25.00 3.00E-02 1.99E-01 1.40E-01
C 8.33 1.49E-01 1.77E-01
D 2.78 1.40E-02 9.10E-02 5.80E-02
E 0.93 4.40E-02 4.90E-02
F 0.31 3.00E-03 1.70E-02 1.20E-02
G 0.10 7.00E-03 6.50E-03
H 0.03 0.00E+00 5.00E-04 1.00E-03
 0.2875      10×

Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by tranisabella and last updated on 2025-04-15 14:32:02.


Thermostability Assay Data

slope of E340L A420 nm (min−1)
T (°C) 1 2 3
A 30.0 0.021 0.02 0.0205
B 31.3 0.02 0.02 0.0195
C 33.6 0.0165 0.016 0.0165
D 37.7 0.007 0.0065 0.0065
E 42.4 0.0005 0.0005 0.0005
F 45.7 0 0
G 48.3 0 0
H 50.0 7.40E-17 0

Protein purified on 2/25/2025 and assayed on 4/1/2025.
Data uploaded by anandsrinivasan and last updated on 2025-04-15 14:48:14.


Reference Data for WT

slope of E340L A420 nm (min−1)
T (°C) 1 2 3
A 30.0 5.70E-02 5.70E-02 5.80E-02
B 31.3 5.80E-02 5.65E-02 5.55E-02
C 33.6 5.80E-02 6.20E-02 6.00E-02
D 37.7 4.65E-02 4.50E-02 4.70E-02
E 42.4 1.80E-02 1.65E-02 1.75E-02
F 45.7 5.00E-04 5.00E-04
G 48.3 5.00E-04 5.00E-04
H 50.0 7.40E-17 0.00E+00

Protein purified on 2/25/25 and assayed on 3/25/25.
Data uploaded by tranisabella and last updated on 2025-04-15 14:09:23.


Design Data

2.926

Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.